Journal Of Parasit
PARASITOLOGY
Saya mendapat dosen parasitologi lulusan UI yang katanya selalu belajar menggunakan jurnal dalam bahasa Inggris, so... my task was also to be the same as she :)
Saya mendapat dosen parasitologi lulusan UI yang katanya selalu belajar menggunakan jurnal dalam bahasa Inggris, so... my task was also to be the same as she :)
- In vivo imaging of trypanosomes for a better assessment of host–parasite relationships and drug efficacy
Abstract: The advances in microscopy combined to the invaluable progress carried by the utilization of molecular, immunological or immunochemical markers and the implementation of more powerful imaging technologies have yielded great improvements to the knowledge of the interaction between microorganisms and their hosts, notably a better understanding of the establishment of infectious processes. Still today, the intricacies of the dialog between parasites, cells and tissues remain limited. Some improvements have been attained with the stable integration and expression of the green fluorescence protein or firefly luciferase and other reporter genes, which have allowed to better approach the monitoring of gene expression and protein localization in vivo, in situ and in real time. Aiming at better exploring the well-established models of murine infections with the characterized strains of Trypanosoma cruzi and Trypanosoma vivax, we revisited in the present report the state of the art about the tools for the imaging of Trypanosomatids in vitro and in vivo and show the latest
transgenic parasites that we have engineered in our laboratory using conventional transfection methods. The targeting of trypanosomes presented in this study is a promising tool for approaching the biology of parasite interactions with host cells, the progression of the diseases they trigger and the screening of new drugs in vivo or in vitro.
click here for download :)
- Pathogenicity of Achlya proliferoides and Saprolegnia diclina
(Saprolegniaceae) Associated with Saprolegniosis Outbreaks in Cultured
Nile Tilapia (Oreochromis niloticus)
(saya menggunakan jurnal ini untuk tugas, eksperimennya mudah dipahami :D )
Abstract: A study was conducted to evaluate the pathogenicity and pathology of Achlya proliferoides
BSN-M005 and Saprolegnia diclina BSN-M003, isolated from saprolegniosis outbreaks against immature stages of Nile tilapia, Oreochromis niloticus. The cumulative mortality rates of the tested fish groups that exposed to high zoospore concentrations of A. proliferoides BSN-M005 and S. diclina BSN-M003 were 60 and 100% respectively. The histopathological changes associated with saprolegniosis lesions induced by S. diclina BSN-M003, were loss of the epidermis, edema of the hypodermis, different degrees of degenerative changes in the underling musculature and the fungal elements were observed penetrating the entire musculature. On the other hand, the histopathological changes associated with saprolegniosis lesions induced by A. proliferoides BSN-M005 showed mats of hyphae attached the surface of epidermis, necrosis, degenerative changes close to hyphae and sometimes fungal elements were observed penetrate into dermal layer but never reach the underling musculature. It is clear from our results that S. diclina BSN-M003 is highly pathogenic than A. proliferoides BSN-M005 to Nile tilapia.
click here for download :) - Monitoring and evaluation of lymphatic filariasis interventions:
an improved PCR-based pool screening method for high throughput
Wuchereria bancrofti detection using dried blood spots
Abstract: Background: Effective diagnostic tools are necessary to monitor and evaluate interruption of Lymphatic Filariasis (LF) transmission. Accurate detection of Wuchereria bancrofti (Wb) microfilaria (mf) is essential to measure the impact of community treatment programmes. PCR-based assays are specific, highly sensitive tools allowing the detection of Wuchereria bancrofti DNA in human blood samples. However, current protocols describing the pool screening approach, use samples of less than 60 μl of blood, which limits the sensitivity of the pool-screen PCR assay. The purpose of this study was to improve the pool-screen PCR protocol to enhance its sensitivity and usefulness for population scale studies. Findings: DNA extractions were performed with the DNeasy kit, the PCR with the Wb LDR primers and the SYBR-Green dye. Improvements of our pool-screen real-time PCR (qPCR) assay allowed the detection of as little as one Wb microfilaria diluted in a pool of at least 12 blood samples of 60 μl each. Using this assay, mf burdens can be predicted using a standard curve derived from mf spiked dried blood samples. The sensitivity achieved is equivalent to the detection of a single LF positive individual carrying a mf burden as low as 18 mf/ml, in a pool of blood samples from at least 12 individuals. Conclusions: Due to its sensitivity, rapidity and cost-effectiveness, we suggest this qPCR pool-screening assay could be used as a diagnostic tool for population- scale filariasis elimination monitoring and evaluation.
click here for download :) - Research Article: New Host Record for Camponotophilus delvarei
(Hymenoptera: Eurytomidae), a Parasitoid of Microdontine Larvae
(Diptera: Syrphidae), Associated with the Ant Camponotus sp. aff. textor
- This is not Journal but Research Article -
click here for download :) - Loa loa microfilaremia in a blood donor—A case report
Abstract: We report a case of Loa loa microfilaremia in a healthy blood donor. The potential for transfu- sion-transmitted infection and the possibility of transfusion-associated allergic reactions calls for stringent donor requirements. We recom- mend that all blood products be additionally screened for possible hemoparasites.
click here for download :)
Nice artikel .........
BalasHapus